Abstract
In this study, our purpose was to explore the synergistic anti-tumor effect and mechanism of paclitaxel nanoparticles (PTX-NPs) combined with radiotherapy (RT) on human cervical carcinoma (HeLa). PTX-NPs were prepared by a solid dispersion method using methoxy poly(ethylene glycol)-poly(ɛ-caprolactone) (MPEG-PCL), which combined with RT exerted a potent and high efficient effect against cervical cancer. In vivo antitumor activity of PTX-NPs combined with RT, was estimated using nude mice carrying Hela cell xenograft tumor. The results were evaluated using microfluorine-18-deoxyglucose PET/computed tomography (18F-FDG PET/CT) and immunohistochemistry. The results showed that PTX-NPs possessed a more efficient effect than PTX when combined with RT (p < 0.05). PTX-NPs in combination with RT might inhibit cell proliferation through its action on Ki-67, and decreased micro-vessel density (MVD) associated with CD31 and vascular endothelial growth factor (VEGF). These results suggested that PTX-NPs possessed a synergistic anti-tumor effect against cervical cancer when combined with RT.
Keywords:
Paclitaxel; cervical cancer; nanoparticle; radiotherapy; synergistic effect.
MeSH terms
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Angiogenesis Inhibitors / administration & dosage
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Angiogenesis Inhibitors / chemistry
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Animals
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Antineoplastic Agents, Phytogenic / administration & dosage*
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Antineoplastic Agents, Phytogenic / chemistry
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Cell Proliferation / drug effects
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Chemoradiotherapy*
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Drug Carriers*
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Drug Compounding
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Female
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Fluorodeoxyglucose F18
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HeLa Cells
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Humans
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Immunohistochemistry
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Ki-67 Antigen / metabolism
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Mice, Inbred BALB C
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Mice, Nude
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Nanomedicine
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Nanoparticles*
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Paclitaxel / administration & dosage*
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Paclitaxel / chemistry
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Platelet Endothelial Cell Adhesion Molecule-1 / metabolism
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Polyesters / chemistry*
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Polyethylene Glycols / chemistry*
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Positron Emission Tomography Computed Tomography
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Radiopharmaceuticals
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Technology, Pharmaceutical / methods
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Time Factors
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Uterine Cervical Neoplasms / diagnostic imaging
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Uterine Cervical Neoplasms / metabolism
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Uterine Cervical Neoplasms / pathology
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Uterine Cervical Neoplasms / therapy*
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Vascular Endothelial Growth Factor A / metabolism
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X-Ray Microtomography
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Xenograft Model Antitumor Assays
Substances
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Angiogenesis Inhibitors
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Antineoplastic Agents, Phytogenic
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Drug Carriers
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Ki-67 Antigen
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Platelet Endothelial Cell Adhesion Molecule-1
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Polyesters
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Radiopharmaceuticals
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VEGFA protein, human
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Vascular Endothelial Growth Factor A
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methoxy poly(ethylene glycol-co-epsilon-caprolactone)
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Fluorodeoxyglucose F18
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Polyethylene Glycols
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Paclitaxel