A new pyrazoline-based probe of quenched fluorescent reversible recognition for Cu2+ and its application in cells

Ying-Peng Zhang; Yu-Ying Dong; Yun-Shang Yang; Hui-Chen Guo; Bi-Xia Cao; Shi-Qi Sun

doi:10.1016/j.saa.2017.01.042

A new pyrazoline-based probe of quenched fluorescent reversible recognition for Cu²⁺ and its application in cells

Spectrochim Acta A Mol Biomol Spectrosc. 2017 Apr 15:177:147-152. doi: 10.1016/j.saa.2017.01.042. Epub 2017 Jan 25.

Authors

Ying-Peng Zhang¹, Yu-Ying Dong², Yun-Shang Yang³, Hui-Chen Guo⁴, Bi-Xia Cao², Shi-Qi Sun⁴

Affiliations

¹ School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China. Electronic address: yingpengzhang@126.com.
² School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
³ School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China. Electronic address: yangguang@lut.cn.
⁴ Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.

PMID: 28153812
DOI: 10.1016/j.saa.2017.01.042

Abstract

A new pyrazoline-based probe D was designed and synthesized, which can be used as a highly sensitive, selective and reversible recognizing fluorescent to detect Cu²⁺. The recognition properties of this compound was investigated by UV-vis absorption and fluorescence spectrophotometry. The results showed that the probe D forms a 1:1 complex with Cu²⁺ and displayed a linear fluorescence response to Cu²⁺ with a detection limit of 1.94×10^-7M. In addition, the probe have a good biocompatibility in living cells.

Keywords: Cu(2+) recognition fluorescence probe living cells.

MeSH terms

Animals
Cell Death
Cell Line
Copper / analysis*
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Pyrazoles / chemistry*
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet

Substances

Fluorescent Dyes
Pyrazoles
Copper