Paper with immobilized protein can provide a low-cost platform for diagnostics, but evidence is emerging that the instability of protein on paper during shipping and storage severely limits its commercial development. Of these, paper-immobilized antibody, though widely used, is in urgent need of improvement in the shelf life. Herein we provided a detailed investigation of a simple and versatile approach for achieving stable antibody on paper to improve bioassay performance. Anti-Blood Group A IgM was chosen as the model antibody and its activity was tested via agglutination reactions. Representative polysaccharides (i.e., chitosan, sodium alginate and dextran) were used as protectants and their ability to stabilize antibody under heat and desiccation stresses was investigated. It was found that the presence of dextran with high concentration markedly stabilized paper-immobilized IgM for at least 120 days. Our results indicate that dextran with good film-forming ability could be a protectant for paper-immobilized antibody.
Keywords: Antibody immobilization; Dextran; Paper-based biosensor; Polysaccharide protection.
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