Light-Triggered Inactivation of Enzymes with Photothermal Nanoheaters

Sebastian A Thompson; Sureyya Paterson; Marwa M M Azab; Alastair W Wark; Roberto de la Rica

doi:10.1002/smll.201603195

Light-Triggered Inactivation of Enzymes with Photothermal Nanoheaters

Small. 2017 Apr;13(15). doi: 10.1002/smll.201603195. Epub 2017 Feb 2.

Authors

Sebastian A Thompson^{1

2}, Sureyya Paterson¹, Marwa M M Azab¹, Alastair W Wark¹, Roberto de la Rica¹

Affiliations

¹ WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Technology and Innovation Centre, 99 George Street, Glasgow, G1 1RD, Scotland, UK.
² Department of Chemistry and Biochemistry, Hunter College-City University of New York, New York, NY, 10065, USA.

PMID: 28151578
DOI: 10.1002/smll.201603195

Abstract

A universal method for inactivating enzymes on demand is introduced, which involves irradiating nanorod-bound enzymes with near-infrared light. The subsequent generation of plasmonic heat denatures the enzymes selectively without damaging other proteins or cell membranes present in the same solution.

Keywords: glucose oxidase; gold; horseradish peroxidase; hyperthermia; nanorods.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CHO Cells
Cricetinae
Cricetulus
Enzyme Activation / radiation effects
Glucose Oxidase / metabolism*
Horseradish Peroxidase / metabolism*
Light*
Nanotubes / chemistry*
Temperature*

Substances

Glucose Oxidase
Horseradish Peroxidase