Abstract
Mitochondria play pivotal roles in the ATP production, apoptosis and generation of reactive oxygen species. Although dynamic regulation of mitochondria morphology is a critical step to maintain cellular homeostasis, the regulatory mechanisms are not yet fully elucidated. In this study, we identified miR-200a-3p as a novel regulator of mitochondrial dynamics by targeting mitochondrial fission factor (MFF). We demonstrated that the ectopic expression of miR-200a-3p enhanced mitochondrial elongation, mitochondrial ATP synthesis, mitochondrial membrane potential and oxygen consumption rate. These results indicate that miR-200a-3p positively regulates mitochondrial elongation by downregulating MFF expression. [BMB Reports 2017; 50(4): 214-219].
MeSH terms
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3' Untranslated Regions
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Adenosine Triphosphate / metabolism
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Antagomirs / metabolism
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Base Sequence
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Cell Line
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Dynamins
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GTP Phosphohydrolases / metabolism
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Humans
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Membrane Potential, Mitochondrial
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Membrane Proteins / antagonists & inhibitors
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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MicroRNAs / antagonists & inhibitors
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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Microscopy, Fluorescence
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Microtubule-Associated Proteins / metabolism
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Mitochondria / metabolism*
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Mitochondrial Dynamics / physiology*
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Mitochondrial Membrane Transport Proteins / metabolism
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Mitochondrial Proteins / antagonists & inhibitors
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Mitochondrial Proteins / genetics
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Mitochondrial Proteins / metabolism*
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Oxygen Consumption
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Sequence Alignment
Substances
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3' Untranslated Regions
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Antagomirs
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Membrane Proteins
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Mff protein, human
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MicroRNAs
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Microtubule-Associated Proteins
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Mitochondrial Membrane Transport Proteins
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Mitochondrial Proteins
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Adenosine Triphosphate
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GTP Phosphohydrolases
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OPA1 protein, human
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Mfn1 protein, human
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DNM1L protein, human
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Dynamins