We proposed a method for generation of a femtosecond optical vortex pulse in a two-mode fiber based on an acoustically induced fiber grating (AIFG) driven by a radio frequency source. Theoretical analysis and experimental results demonstrated that the left- and right-handed circular polarization fundamental modes of the femtosecond optical pulse could be converted to the linearly polarized ±1-order optical vortex modes through the AIFG with the mode conversion efficiency of ∼95%. The off-axial interference experiment and the polarization angle-dependent intensity examination were performed to verify the topological charge and the polarization state of the femtosecond optical vortex, respectively.