First genotype identification of Trichosporon asahii in Sfax, Tunisia

J Med Microbiol. 2017 Apr;66(4):397-401. doi: 10.1099/jmm.0.000442. Epub 2017 Apr 28.

Abstract

Purpose: The objectives of our study were species identification and genotyping of Trichosporon isolates collected at the Parasitology and Mycology Laboratory in Sfax, Tunisia.

Methodology: Molecular identification was carried out by analysing the IGS1 regions of the rDNA of 30 Trichosporon isolates.

Results: Trichosporon asahii was the most frequent species detected. Furthermore, four genotypes were identified in Tunisia: 1 (46.4 %), 4 (35.7 %), 7 (14.3 %) and 3 (3.6 %). In vitro antifungal susceptibility testing of the isolates showed that voriconazole exhibited the highest activity.

Conclusion: This is the first reported study of genotype identification of T. asahii in Tunisia and even in the African continent.

MeSH terms

  • Antifungal Agents / pharmacology*
  • DNA, Intergenic / genetics*
  • DNA, Ribosomal / genetics
  • Genotype
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Typing
  • Mycological Typing Techniques
  • RNA, Ribosomal, 28S / genetics
  • Trichosporon / classification
  • Trichosporon / drug effects*
  • Trichosporon / genetics*
  • Trichosporon / isolation & purification
  • Tunisia
  • Voriconazole / pharmacology*

Substances

  • Antifungal Agents
  • DNA, Intergenic
  • DNA, Ribosomal
  • RNA, Ribosomal, 28S
  • Voriconazole