A protocol to produce synthetic oats by introducing alien genetic variations into cultivated oats is proposed based on suitable genetic relationships between Avena species. Furthermore, artificial hybridization procedures between different ploidy species are explained. Amphiploids can be produced by rescuing aborted embryos and treating the F1 hybrids with colchicine to overcome sterility between interspecific plants. Furthermore, I present the cytological methods for observing somatic and meiotic chromosomes in the treated hybrids.
Keywords: Amphiploid; Avena sativa; Chromosome doubling; Colchicine; Emasculation; Embryo rescue; Hybridization; Pollination; Polyploid; Synthetic oats.