Arabidopsis MAPKKK18 positively regulates drought stress resistance via downstream MAPKK3

Biochem Biophys Res Commun. 2017 Mar 4;484(2):292-297. doi: 10.1016/j.bbrc.2017.01.104. Epub 2017 Jan 25.

Abstract

Mitogen-activated protein kinase (MAPK) cascades are conserved and vital signaling components in the responses to various ambient stresses. Here, we report the identification of MAPKKK18, a drought resistance associated MAPK kinase kinase in Arabidopsis. The mapkkk18 knockout mutants displayed hypersensitivity to drought stress, whereas overaccumulation of MAPKKK18 in transgenic Arabidopsis plants significantly enhanced the resistance to drought. Expression pattern analysis revealed that the inducible expression of MAPKKK18 by osmotic stress was ABA and the canonical ABA signaling pathway dependent. Furthermore, MAPKKK18 mainly exerted its regulatory roles via downstream MAPKK3. These findings uncovered important roles for MAPKKK18 in drought resistance and expanded our understanding of the MAPK pathways in modulating abiotic stress responses.

Keywords: Arabidopsis; Drought stress; MAPK; MAPKK3; MAPKKK18.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / metabolism
  • Arabidopsis / enzymology
  • Arabidopsis / physiology*
  • Arabidopsis Proteins / physiology*
  • Droughts*
  • MAP Kinase Kinase Kinases / physiology*
  • Mitogen-Activated Protein Kinases / metabolism*
  • Osmotic Pressure
  • Plant Stomata
  • Stress, Physiological*

Substances

  • Arabidopsis Proteins
  • Abscisic Acid
  • Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase Kinases
  • MAPKKK18 protein, Arabidopsis