Abstract
Identification of related mutants contributed a lot to our understanding of the molecular mechanisms underlying brassinosteroid (BR) signaling. Establishment of methods to test for the hormone sensitivity is essential for the characterization of the mutants associated with BR responses. Here, we describe detailed procedures for the physiological analysis of BR responses that are regularly applied to test the BR sensitivity of mutants by evaluating the growth of different tissues of rice (Oryza sativa) seedlings.
Keywords:
Brassinosteroid response; Brassinosteroid sensitivity; Coleoptile elongation; Lamina inclination; Rice.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Brassinosteroids / pharmacology*
-
Cotyledon / drug effects
-
Cotyledon / genetics
-
Cotyledon / growth & development
-
Cotyledon / metabolism
-
Gene Expression Regulation, Developmental
-
Gene Expression Regulation, Plant*
-
Mutation
-
Oryza / drug effects*
-
Oryza / genetics
-
Oryza / growth & development
-
Oryza / metabolism
-
Plant Growth Regulators / pharmacology*
-
Plant Leaves / drug effects
-
Plant Leaves / genetics
-
Plant Leaves / growth & development
-
Plant Leaves / metabolism
-
Plant Proteins / genetics*
-
Plant Proteins / metabolism
-
Plant Roots / drug effects
-
Plant Roots / genetics
-
Plant Roots / growth & development
-
Plant Roots / metabolism
-
Seedlings / drug effects
-
Seedlings / genetics
-
Seedlings / growth & development
-
Seedlings / metabolism
-
Signal Transduction
-
Steroids, Heterocyclic / pharmacology*
Substances
-
Brassinosteroids
-
Plant Growth Regulators
-
Plant Proteins
-
Steroids, Heterocyclic
-
brassinolide