Lanthanide-to-quantum dot Förster resonance energy transfer (FRET): Application for immunoassay

O A Goryacheva; N V Beloglazova; A M Vostrikova; M V Pozharov; A M Sobolev; I Yu Goryacheva

doi:10.1016/j.talanta.2016.11.054

Lanthanide-to-quantum dot Förster resonance energy transfer (FRET): Application for immunoassay

Talanta. 2017 Mar 1:164:377-385. doi: 10.1016/j.talanta.2016.11.054. Epub 2016 Nov 30.

Authors

O A Goryacheva¹, N V Beloglazova², A M Vostrikova¹, M V Pozharov¹, A M Sobolev¹, I Yu Goryacheva³

Affiliations

¹ Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia.
² Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia; Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
³ Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia; Department of Analytical Chemistry Chemistry Institute, St.Petersburg State University, Universitetsky pr. 198504, St.Petersburg, Russia. Electronic address: goryachevaiy@mail.ru.

PMID: 28107944
DOI: 10.1016/j.talanta.2016.11.054

Abstract

Förster resonance energy transfer (FRET) between lanthanide ion complexes (L) acting as donors and luminescent semiconductor quantum dots (QD) acting as acceptors is discussed in the terms of advantages and disadvantages for its application in immunoassay. L-QD-FRET is potentially a powerful tool that can be used to detect and confirm formation of immunocomplexes, but until now it had very limited practical analytical application. Therefore, the main aim of this review is to analyze all possibilities, advantages, and disadvantages of L-QD-FRET in immunoassay applications. Considering L and QD respectively applied as donor and acceptor, the most advantageous properties for analytical purposes are large decay time of L complexes and the high absorption of QD. L complexes' extremely long decay times make it possible to directly detect FRET through enhancement of QDs decay time as a result of energy transfer. Very high QD absorption predetermines extremely large Förster radii (ca. 10nm), which means that FRET can be utilized for proteins and protein complexes, such as antigen-antibody systems.

Keywords: Förster resonance energy transfer (FRET); Lanthanide ion complex; Quantum dot, immunoassay, antigen-antibody interaction.

Publication types

Review