Copper(II)-Controlled Molecular Glue for Mismatched DNA

Chembiochem. 2017 Apr 4;18(7):618-622. doi: 10.1002/cbic.201600675. Epub 2017 Feb 20.

Abstract

Isothermal hybridization of two DNA strands bearing three thymine-thymine (T:T) mismatches can be brought about in the presence of a stoichiometric amount of a bis-naphthalene macrocycle, 2,7-BisNP-NH. This process can be reverted by addition of a CuII salt due to formation of a dinuclear metal complex which does not bind to DNA. Subsequent sequestration of CuII releases the macrocycle and restores the hybridization state of DNA strands, thus allowing implementation of a fast fluorescent two-state DNA switch.

Keywords: DNA recognition; DNA switch; hybridization; macrocycles; metal complexes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pair Mismatch
  • Coordination Complexes / chemistry*
  • Copper / chemistry*
  • DNA / chemistry*
  • Edetic Acid / chemistry
  • Intercalating Agents / chemistry*
  • Ligands
  • Macrocyclic Compounds / chemistry*
  • Naphthalenes / chemistry*
  • Nucleic Acid Denaturation
  • Nucleic Acid Hybridization
  • Perchlorates / chemistry*
  • Temperature
  • Thymine / chemistry

Substances

  • Coordination Complexes
  • Intercalating Agents
  • Ligands
  • Macrocyclic Compounds
  • Naphthalenes
  • Perchlorates
  • copper(II) perchlorate
  • Copper
  • DNA
  • Edetic Acid
  • Thymine