Abstract
We investigated the effect of Dalbergioidin (DAL), a well-known natural product extracted from Uraria crinita, on doxorubicin- (DXR-) induced renal fibrosis in mice. The mice were pretreated for 7 days with DAL followed by a single injection of DXR (10 mg/kg) via the tail vein. Renal function was analyzed 5 weeks after DXR treatment. DXR caused nephrotoxicity. The symptoms of nephrotic syndrome were greatly improved after DAL treatment. The indices of renal fibrosis, the phosphorylation of Smad3, and the expression of alpha-smooth muscle actin (α-SMA), fibronectin, collagen III (Col III), E-cadherin, TGF-β, and Smad7 in response to DXR were all similarly modified by DAL. The present findings suggest that DAL improved the markers for kidney damage investigated in this model of DXR-induced experimental nephrotoxicity.
MeSH terms
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Actins / metabolism
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Animals
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Antibiotics, Antineoplastic / adverse effects
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Cadherins / metabolism
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Chromones / therapeutic use*
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Collagen Type III / metabolism
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Doxorubicin / adverse effects*
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Fibronectins / metabolism
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Fibrosis / chemically induced
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Fibrosis / drug therapy*
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Glutathione / metabolism
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Kidney / drug effects*
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Kidney / pathology
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Kidney Diseases / metabolism
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Mice
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Muscle, Smooth / metabolism
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Neuroprotective Agents / therapeutic use
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Oxidation-Reduction
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Phosphorylation
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Plant Extracts / therapeutic use
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Random Allocation
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Signal Transduction
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Smad3 Protein / metabolism
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Smad7 Protein / metabolism
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Transforming Growth Factor beta / antagonists & inhibitors*
Substances
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Actins
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Antibiotics, Antineoplastic
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Cadherins
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Chromones
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Collagen Type III
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Fibronectins
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Neuroprotective Agents
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Plant Extracts
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Smad3 Protein
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Smad3 protein, mouse
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Smad7 Protein
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Smad7 protein, mouse
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Transforming Growth Factor beta
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dalbergioidin
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Doxorubicin
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Glutathione