We demonstrate a combined printing process utilizing inkjet printing of silver electrodes and solid-ink technology for printing hydrophobic wax barriers for fabricating paper microfluidic devices with integrated electrodes. Optimized printing parameters are given for achieving conducting silver lines on the top of macroporous chromatography paper down to 250 μm-300 μm resolution. Electrical characterization and wicking experiments demonstrate that the printed silver patterns are simultaneously conductive and porous enough to allow reliable capillary wicking across the electrodes. The combined wax and silver printing method is used for fabrication of paper microfluidic isoelectric focusing devices for separation and concentration of proteins.