An antimicrobial peptide with an aggregation-induced emission (AIE) luminogen for studying bacterial membrane interactions and antibacterial actions

Chem Commun (Camb). 2017 Mar 16;53(23):3315-3318. doi: 10.1039/c6cc09408b.

Abstract

A fluorescence technique to investigate the interactions between bacterial membranes and an aggregation-induced emission (AIE) luminogen-decorated AMP (TPE-AMP) was reported. Our simple and fast method consists of mixing TPE-AMP and bacterial suspensions and recording the fluorescence signals by flow cytometry and confocal microscopy in a "non-washing" manner.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / metabolism*
  • Anti-Bacterial Agents / radiation effects
  • Anti-Bacterial Agents / toxicity
  • Antimicrobial Cationic Peptides / chemistry
  • Antimicrobial Cationic Peptides / metabolism*
  • Antimicrobial Cationic Peptides / radiation effects
  • Antimicrobial Cationic Peptides / toxicity
  • Cell Membrane / chemistry
  • Cell Membrane / metabolism*
  • Escherichia coli / drug effects
  • Fluorescence
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism*
  • Fluorescent Dyes / radiation effects
  • Fluorescent Dyes / toxicity
  • Methacrylates / chemistry
  • Methacrylates / metabolism*
  • Methacrylates / radiation effects
  • Methacrylates / toxicity
  • Mice, Inbred ICR
  • Microbial Sensitivity Tests
  • Protein Binding
  • Pseudomonas aeruginosa / drug effects
  • Staphylococcus aureus / drug effects
  • Staphylococcus epidermidis / drug effects
  • Ultraviolet Rays

Substances

  • Anti-Bacterial Agents
  • Antimicrobial Cationic Peptides
  • Fluorescent Dyes
  • Methacrylates