Measuring abscission spatiotemporal dynamics using quantitative high-resolution microscopy

O Gershony; S Sherman; S Adar; I Segal; D Nachmias; I Goliand; N Elia

doi:10.1016/bs.mcb.2016.03.032

Measuring abscission spatiotemporal dynamics using quantitative high-resolution microscopy

Methods Cell Biol. 2017:137:205-224. doi: 10.1016/bs.mcb.2016.03.032. Epub 2016 Jul 9.

Authors

O Gershony¹, S Sherman¹, S Adar¹, I Segal¹, D Nachmias¹, I Goliand¹, N Elia¹

Affiliation

¹ Ben Gurion University of the Negev, Beer Sheva, Israel.

PMID: 28065306
DOI: 10.1016/bs.mcb.2016.03.032

Abstract

The spatiotemporal characteristics of ESCRT (Endosomal Sorting Complex Required for Transport)-mediated mammalian cytokinetic abscission have been studied in recent years using quantitative high-resolution light microscopy techniques. Here we describe how to apply spinning disk live cell imaging and structured illumination microscopy (SIM) to define the dynamics and structural organization of abscission and of proteins involved in abscission in a quantitative manner. We further provide a protocol to correlate the structural data, obtained by SIM, to the dynamic information obtained by live cell recordings.

Keywords: Cell cycle; Cell division; Cytokinesis; ESCRT; Intercellular bridge; Live cell imaging; Membrane fission; Structured illumination microscopy.

MeSH terms

Cytokinesis / genetics*
Endosomal Sorting Complexes Required for Transport / genetics
Endosomal Sorting Complexes Required for Transport / ultrastructure
Endosomes / genetics
Endosomes / ultrastructure*
HeLa Cells
Humans
Microscopy / methods*
Molecular Imaging / methods*

Substances

Endosomal Sorting Complexes Required for Transport