Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer

E I Shramova; G M Proshkina; S P Chumakov; Yu M Khodarovich; S M Deyev

Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer

Acta Naturae. 2016 Oct-Dec;8(4):118-123.

Authors

E I Shramova¹, G M Proshkina¹, S P Chumakov¹, Yu M Khodarovich¹, S M Deyev²

Affiliations

¹ Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997, Moscow, Russia.
² Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997, Moscow, Russia ; National Research Tomsk Polytechnic University, Lenina av., 30, 634050, Tomsk, Russia.

PMID: 28050273
PMCID: PMC5199213

Abstract

In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, furimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells in fusion with NanoLuc luciferase is activated in the presence of its substrate, furimazine. Upon such condition, miniSOG possesses photoinduced cytotoxicity and causes a 48% cell death level in a stably transfected cell line.

Keywords: NanoLuc luciferase; bioluminescence resonance energy transfer; flavoprotein miniSOG; photodynamic therapy.