An anticoagulant-active polysaccharide PF2 was extracted with boiling water from the green seaweed Monostroma angicava, further purified by anion-exchange and size-exclusion chromatography. PF2 was a rhamnan-type sulfated polysaccharide with molecular weight of about 88.1kDa. Results of chemical and spectroscopic analyses demonstrated that PF2 consisted of→3)-α-l-Rhap-(1→ and →2)-α-l-Rhap-(1→residues, with partially branches at C-2 of→3)-α-l-Rhap-(1→residues. Sulfate groups were substituted at C-3 of →2)-α-l-Rhap-(1→ residues. The sulfated polysaccharide PF2 had a high anticoagulant action, and the mechanism of anticoagulant activity mediated by PF2 was mainly attributed to strong potentiation thrombin by heparin cofactor II. PF2 also exhibited weak effect on antithrombin-dependent thrombin or factor Xa inhibition. The fibrin(ogen)olytic activity and thrombolytic activity of PF2 were also evaluated. The investigation revealed that PF2 was a novel sulfated rhamnan differing from previously described sulfated polysaccharides from green seaweed and could be a potential anticoagulant polysaccharide.
Keywords: Acetonitrile (PubChem CID: 6342); Anticoagulant activity; Barium rhodizonic acid (PubChem CID: 45048930); Dimethyl sulfoxide (PubChem CID: 679); Heparin (PubChem CID: 25244225); Inhibition mechanism; Iodomethane (PubChem CID: 6328); Monostroma angicava; Pullunan (PubChem CID: 92024139); Pyridine (PubChem CID: 1049); Rhamnose (PubChem CID: 25310); Structure; Sulfated polysaccharide; d-Glucuronic acid (PubChem CID: 94715); l-Phenyl-3-methyl-5-pyrazolone (PubChem CID: 90474051).
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