The pre-existing population of 5S rRNA effects p53 stabilization during ribosome biogenesis inhibition

Oncotarget. 2017 Jan 17;8(3):4257-4267. doi: 10.18632/oncotarget.13833.

Abstract

Pre-ribosomal complex RPL5/RPL11/5S rRNA (5S RNP) is considered the central MDM2 inhibitory complex that control p53 stabilization during ribosome biogenesis inhibition. Despite its role is well defined, the dynamic of 5S RNP assembly still requires further characterization. In the present work, we report that MDM2 inhibition is dependent by a pre-existing population of 5S rRNA.

Keywords: 5S rRNA; MDM2; p53; ribosomal proteins; ribosome biogenesis inhibition.

MeSH terms

  • A549 Cells
  • HCT116 Cells
  • Humans
  • MCF-7 Cells
  • Protein Stability
  • Proto-Oncogene Proteins c-mdm2 / metabolism
  • RNA, Ribosomal, 5S / genetics*
  • RNA, Ribosomal, 5S / metabolism
  • Ribosomal Proteins / metabolism*
  • Ribosomes / metabolism*
  • Transcription Factor TFIIIA / metabolism
  • Transcription, Genetic
  • Tumor Suppressor Protein p53 / chemistry*

Substances

  • RNA, Ribosomal, 5S
  • Ribosomal Proteins
  • TP53 protein, human
  • Transcription Factor TFIIIA
  • Tumor Suppressor Protein p53
  • ribosomal protein L11
  • ribosomal protein L5, human
  • MDM2 protein, human
  • Proto-Oncogene Proteins c-mdm2