High-speed photoacoustic microscopy of mouse cortical microhemodynamics

Li Lin; Junjie Yao; Ruiying Zhang; Chun-Cheng Chen; Chih-Hsien Huang; Yang Li; Lidai Wang; William Chapman; Jun Zou; Lihong V Wang

doi:10.1002/jbio.201600236

High-speed photoacoustic microscopy of mouse cortical microhemodynamics

J Biophotonics. 2017 Jun;10(6-7):792-798. doi: 10.1002/jbio.201600236. Epub 2016 Dec 23.

Authors

Li Lin¹, Junjie Yao^{1

2}, Ruiying Zhang¹, Chun-Cheng Chen³, Chih-Hsien Huang⁴, Yang Li¹, Lidai Wang^{1

5}, William Chapman³, Jun Zou⁴, Lihong V Wang¹

Affiliations

¹ Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA.
² Department of Biomedical Engineering, Duke University, Durham, NC, USA.
³ Department of Surgery, Washington University School of Medicine, St. Louis, MO, USA.
⁴ Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX, USA.
⁵ Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong.

Abstract

We applied high-speed photoacoustic microscopy (PAM) for both cortical microenvironment studies and dynamic brain studies, with micrometer-level optical resolution and a millisecond-level cross-sectional imaging speed over a millimeter-level field of view. We monitored blood flow redistribution in mini-stroke mouse models and cerebral autoregulation induced by a vasoactive agent. Our results collectively suggest that high-speed PAM is a promising tool for understanding dynamic neurophysiological phenomena, complementing conventional imaging modalities.

Keywords: MEMS scanning mirror; blood flow redistribution; cerebral autoregulation; cortical hemodynamics; microhemorrhage; photoacoustic microscopy.

MeSH terms

Animals
Brain / diagnostic imaging*
Female
Mice
Microscopy*
Photoacoustic Techniques*
Spectrum Analysis

Abstract

MeSH terms

Grants and funding