In social animals, signals released from fearless conspecifics attenuate fear responses, namely social buffering. The presence of conspecific odor can suppress the expression of freezing response of conditioned mice. The present study investigated if physical social experience is required for this social buffering effect. The mice were exposed to donors, donor bedding (collected from cages of donors), or fresh bedding as control, respectively, for 10 days (1 hour daily) in prior to fear conditioning test. The fear expression test was examined in presence of donor bedding. The results showed that only the donor group mice showed reduced freezing time than the other two groups in the fear memory test. This phenomenon indicated that physical interaction might be required for the social buffering effect.