Zwitterionic Iron Oxide (γ-Fe2 O3 ) Nanoparticles Based on P(2VP-grad-AA) Copolymers

Mark Billing; Christine Gräfe; Adrian Saal; Philip Biehl; Joachim H Clement; Silvio Dutz; Steffen Weidner; Felix H Schacher

doi:10.1002/marc.201600637

Zwitterionic Iron Oxide (γ-Fe₂ O₃ ) Nanoparticles Based on P(2VP-grad-AA) Copolymers

Macromol Rapid Commun. 2017 Feb;38(4). doi: 10.1002/marc.201600637. Epub 2016 Dec 22.

Authors

Mark Billing^{1

2}, Christine Gräfe³, Adrian Saal^{1

2}, Philip Biehl^{1

2}, Joachim H Clement^{2

3}, Silvio Dutz⁴, Steffen Weidner⁵, Felix H Schacher^{1

2}

Affiliations

¹ Laboratory of Organic and Macromolecular Chemistry, Friedrich-Schiller-University Jena, Humboldtstraße 10, D-07743, Jena, Germany.
² Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University Jena, Philosophenweg 7, D-07743, Jena, Germany.
³ Klinik für Innere Medizin II, Abteilung Hämatologie und Internistische Onkologie, Universitätsklinikum Jena, Am Klinikum 1, D.07747, Jena, Germany.
⁴ Institute of Biomedical Engineering and Informatics, Technische Universität Ilmenau, Gustav-Kirchhoff-Str. 2, D.98693, Ilmenau, Germany.
⁵ Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Str. 11, D-12489, Berlin, Germany.

PMID: 28004458
DOI: 10.1002/marc.201600637

Abstract

This study presents the synthesis and characterization of zwitterionic core-shell hybrid nanoparticles consisting of a core of iron oxide multicore nanoparticles (MCNPs, γ-Fe₂ O₃ ) and a shell of sultonated poly(2-vinylpyridine-grad-acrylic acid) copolymers. The gradient copolymers are prepared by reversible addition fragmentation chain transfer polymerization of 2-vinylpyridine (2VP), followed by the addition of tert-butyl acrylate and subsequent hydrolysis. Grafting of P(2VP-grad-AA) onto MCNP results in P(2VP-grad-AA)@MCNP, followed by quaternization using 1,3-propanesultone-leading to P(2VP_S -grad-AA)@MCNP with a zwitterionic shell. The resulting particles are characterized by transmission electron microscopy, dynamic light scattering, and thermogravimetric analysis measurements, showing particle diameters of ≈70-90 nm and an overall content of the copolymer shell of ≈10%. Turbidity measurements indicate increased stability toward secondary aggregation after coating if compared to the pristine MCNP and additional cytotoxicity tests do not reveal any significant influence on cell viability.

Keywords: block copolymer; controlled radical polymerization; hybrid nanoparticles; iron oxide nanoparticles; sulfobetaines.

MeSH terms

Acrylic Resins / chemical synthesis
Acrylic Resins / chemistry*
Ferric Compounds / chemistry*
Molecular Structure
Nanoparticles / chemistry*
Particle Size
Polyvinyls / chemical synthesis
Polyvinyls / chemistry*
Surface Properties

Substances

Acrylic Resins
Ferric Compounds
Polyvinyls
ferric oxide