Modified Carba NP test for the detection of carbapenemase production in gram-negative rods: optimized handling of multiple samples

Eloiza H Campana; Stephanie G Chuster; Isadora R da Silva; Raphael P Paschoal; Raquel R Bonelli; Beatriz M Moreira; Renata C Picão

doi:10.1016/j.bjm.2016.09.015

Modified Carba NP test for the detection of carbapenemase production in gram-negative rods: optimized handling of multiple samples

Braz J Microbiol. 2017 Apr-Jun;48(2):242-245. doi: 10.1016/j.bjm.2016.09.015. Epub 2016 Dec 7.

Authors

Eloiza H Campana¹, Stephanie G Chuster², Isadora R da Silva², Raphael P Paschoal², Raquel R Bonelli², Beatriz M Moreira², Renata C Picão²

Affiliations

¹ Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Laboratório de Investigação em Microbiologia Médica, Rio de Janeiro, Brazil. Electronic address: elocampana@gmail.com.
² Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Laboratório de Investigação em Microbiologia Médica, Rio de Janeiro, Brazil.

Abstract

The modified Carba NP test presented here may be a valuable tool for laboratories interested in investigating a large number of carbapenemase-producing bacteria in a less-costly way. The test was evaluated against 48 carbapenemase-producing and carbapenemase-non-producing gram-negative bacteria. No false-positive results were obtained, but false-negative results were observed with OXA-23- and GES-carbapenemase-producing isolates. Aeromonas sp. are not testable by Modified Carba NP.

Keywords: Carbapenemases; Diagnostics; Gram-negative bacteria.

Publication types

Evaluation Study

MeSH terms

Bacterial Proteins / analysis*
Bacteriological Techniques / methods*
False Negative Reactions
Gram-Negative Bacteria / enzymology*
beta-Lactamases / analysis*

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase