Characterization and complete genome sequence of a panicovirus from Bermuda grass by high-throughput sequencing

Muhammad N Tahir; Ben Lockhart; Samuel Grinstead; Dimitre Mollov

doi:10.1007/s00705-016-3165-4

Characterization and complete genome sequence of a panicovirus from Bermuda grass by high-throughput sequencing

Arch Virol. 2017 Apr;162(4):1099-1102. doi: 10.1007/s00705-016-3165-4. Epub 2016 Dec 19.

Authors

Muhammad N Tahir¹, Ben Lockhart², Samuel Grinstead¹, Dimitre Mollov³

Affiliations

¹ USDA, ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA.
² Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA.
³ USDA, ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA. dimitre.mollov@ars.usda.gov.

PMID: 27995336
DOI: 10.1007/s00705-016-3165-4

Abstract

Bermuda grass samples were examined by transmission electron microscopy and 28-30 nm spherical virus particles were observed. Total RNA from these plants was subjected to high-throughput sequencing (HTS). The nearly full genome sequence of a panicovirus was identified from one HTS scaffold. Sanger sequencing was used to confirm the HTS results and complete the genome sequence of 4404 nt. This virus was provisionally named Bermuda grass latent virus (BGLV). Its predicted open reading frames follow the typical arrangement of the genus Panicovirus. Based on sequence comparisons and phylogenetic analyses BGLV differs from other viruses and therefore taxonomically it is a new member of the genus Panicovirus, family Tombusviridae.

MeSH terms

Base Sequence
Genome, Viral*
High-Throughput Nucleotide Sequencing
Molecular Sequence Data
Open Reading Frames
Phylogeny
Plant Diseases / virology
Poaceae / virology*
RNA, Viral
Tombusviridae / classification
Tombusviridae / genetics*
Tombusviridae / isolation & purification
Viral Proteins / genetics

Substances

RNA, Viral
Viral Proteins