Various Sequence Types of Escherichia coli Isolates Coharboring blaNDM-5 and mcr-1 Genes from a Commercial Swine Farm in China

Antimicrob Agents Chemother. 2017 Feb 23;61(3):e02167-16. doi: 10.1128/AAC.02167-16. Print 2017 Mar.

Abstract

Sixteen different sequence types (STs) of Escherichia coli isolates from a commercial swine farm in China were confirmed to coharbor the carbapenem resistance gene blaNDM-5 and the colistin resistance gene mcr-1 Whole-genome sequencing revealed that blaNDM-5 and mcr-1 were located on a 46-kb IncX3 plasmid and a 32-kb IncX4 plasmid, respectively. The two plasmids can transfer together with a low fitness cost, which might explain the presence of various STs of E. coli coharboring blaNDM-5 and mcr-1.

Keywords: Escherichia coli; blaNDM-5; carbapenems; colistin; fitness cost; mcr-1.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Carbapenems / pharmacology
  • China / epidemiology
  • Colistin / pharmacology
  • Drug Resistance, Multiple, Bacterial / genetics*
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Escherichia coli / growth & development
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / epidemiology
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / veterinary*
  • Escherichia coli Proteins / genetics*
  • Escherichia coli Proteins / metabolism
  • Gene Expression
  • Gene Transfer, Horizontal
  • Genetic Fitness
  • Genotype
  • Plasmids / chemistry
  • Plasmids / genetics
  • Plasmids / metabolism
  • Swine
  • Swine Diseases / epidemiology*
  • Swine Diseases / microbiology
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Carbapenems
  • Escherichia coli Proteins
  • MCR-1 protein, E coli
  • beta-Lactamases
  • Colistin