cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans

Han Wang; Jonathan Liu; Shahla Gharib; Cynthia M Chai; Erich M Schwarz; Navin Pokala; Paul W Sternberg

doi:10.1038/nmeth.4109

cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans

Nat Methods. 2017 Feb;14(2):145-148. doi: 10.1038/nmeth.4109. Epub 2016 Dec 19.

Authors

Han Wang^{1

2}, Jonathan Liu^{1

2}, Shahla Gharib^{1

2}, Cynthia M Chai^{1

2}, Erich M Schwarz^{1

2}, Navin Pokala³, Paul W Sternberg^{1

2}

Affiliations

¹ Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
² Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, USA.
³ Department of Life Sciences, New York Institute of Technology, Old Westbury, New York, USA.

Abstract

The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Animals
Animals, Genetically Modified
Caenorhabditis elegans / genetics*
DNA-Binding Proteins / genetics*
Defecation / genetics
Gene Expression Regulation*
Genetic Engineering / methods*
Herpesvirus 1, Human / genetics
Microscopy, Fluorescence
Optogenetics
Saccharomyces cerevisiae Proteins / genetics*
Temperature
Transcription Factors / genetics*

Substances

DNA-Binding Proteins
GAL4 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Transcription Factors

Abstract

Publication types

MeSH terms

Substances

Grants and funding