MALDI-TOF mass spectrometry for rapid diagnosis of postoperative endophthalmitis

Adriane Mailhac; Harmonie Durand; Sandrine Boisset; Danièle Maubon; Francois Berger; Max Maurin; Christophe Chiquet; Marie Bidart

doi:10.1016/j.jprot.2016.10.017

MALDI-TOF mass spectrometry for rapid diagnosis of postoperative endophthalmitis

J Proteomics. 2017 Jan 30:152:150-152. doi: 10.1016/j.jprot.2016.10.017. Epub 2016 Oct 29.

Authors

Adriane Mailhac¹, Harmonie Durand², Sandrine Boisset³, Danièle Maubon⁴, Francois Berger², Max Maurin³, Christophe Chiquet¹, Marie Bidart⁵

Affiliations

¹ Department of Ophthalmology, Grenoble Alpes University Hospital, France.
² Braintech Lab, UMR1205, Grenoble Alpes University, France.
³ Bacteriological Laboratory, Department of Infectious Agents, Biology Institute, Grenoble Alpes University Hospital, France.
⁴ Parasitology and Mycology Laboratory, Department of Infectious Agents, Biology Institute, Grenoble Alpes University Hospital, France; TIMC-IMAG-TheREx, UMR 5525 CNRS-UGA, Grenoble Alpes University, France.
⁵ Braintech Lab, UMR1205, Grenoble Alpes University, France; Clinical Proteomic and Transcriptomic Plateform, Research Department, Grenoble Alpes University Hospital, France; Biochemistry and Genetic Laboratory, Department of Biochemistry, Toxicology and Pharmacology, Biology Institute, Grenoble Alpes University, France. Electronic address: MBidart@chu-grenoble.fr.

PMID: 27989942
DOI: 10.1016/j.jprot.2016.10.017

Abstract

This study describes an innovative strategy for rapid detection and identification of bacteria causing endophthalmitis, combining the use of an automated blood culture system with MALDI-TOF mass spectrometry methodology. Using this protocol, we could identify 96% of 45 bacterial strains isolated from vitreous samples collected in acute post-operative endophthalmitis patients.

Keywords: Blood culture; Direct identification; Endophthalmitis; MALDI-TOF mass spectrometry; Staphylococcus; Vitreous.

MeSH terms

Bacteria / isolation & purification*
Bacteriological Techniques / methods
Endophthalmitis / diagnosis*
Endophthalmitis / microbiology*
High-Throughput Screening Assays
Humans
Postoperative Complications / diagnosis
Postoperative Complications / microbiology*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Vitreous Body / microbiology