Abstract
We developed a nitroreductase responsive theranostic probe 1; it comprises biotinylated rhodol in conjunction with p-nitrobenzyl functionality. The probe 1 showed a remarkable fluorescence 'turn-on' signal in the presence of nitroreductase under physiological conditions. The probe is considerably stable within a wide biological pH range (6-8) and also is very sensitive toward a reducing micro-environment e.g. liver microsome. Further, it enables providing cellular and in vivo nematode images in a reducing microenvironment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biotin / analogs & derivatives*
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Biotin / chemical synthesis
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Biotin / pharmacology*
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Biotin / toxicity
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Caenorhabditis elegans
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Cell Line, Tumor
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Fluorescence
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / pharmacology*
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Fluorescent Dyes / toxicity
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Humans
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Hypoxia / diagnostic imaging*
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Lepidoptera
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Limit of Detection
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Microsomes, Liver / metabolism
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Nitroreductases / metabolism*
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Piperazines / chemical synthesis
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Piperazines / chemistry
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Piperazines / pharmacology
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Piperazines / toxicity
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Rats
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Theranostic Nanomedicine
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Xanthones / chemical synthesis
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Xanthones / chemistry
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Xanthones / pharmacology*
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Xanthones / toxicity
Substances
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Fluorescent Dyes
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Piperazines
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Xanthones
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rhodol
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Biotin
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Nitroreductases