The application of microbeads to microfluidic systems for enhanced detection and purification of biomolecules

Methods. 2017 Mar 1:116:112-124. doi: 10.1016/j.ymeth.2016.12.005. Epub 2016 Dec 10.

Abstract

This paper describes microbead-based microfluidic systems. Several aspects of bead assays in microfluidics make them advantageous for bioassays in simple microchannels, including enhanced surface-to-volume ratio, improved molecular recognition reaction efficiency, and the wide range of surface functionalization available with commercial microbeads. Two-level SU-8 molds are used to fabricate PDMS microchannels that can hydrodynamically trap different types of microbeads, with characteristic dimensions of tens of microns. The use of these microbead-based microfluidic systems in the biosensing of antibodies, toxins and nucleic acids, as well as in antibody purification will be presented and discussed in this paper.

Keywords: Antibodies; Biosensing; Microbeads; Microfluidics; Mycotoxins; Nucleic acids.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / isolation & purification*
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Dimethylpolysiloxanes / chemistry
  • Equipment Design
  • Fluorescent Dyes / chemistry
  • Humans
  • Microfluidic Analytical Techniques / instrumentation*
  • Microspheres
  • Mycotoxins / isolation & purification*
  • Nucleic Acids / isolation & purification*

Substances

  • Antibodies
  • Dimethylpolysiloxanes
  • Fluorescent Dyes
  • Mycotoxins
  • Nucleic Acids
  • baysilon