Dataset of the absorption, emission and excitation spectra and fluorescence intensity graphs of fluorescent cyanine dyes for the quantification of low amounts of dsDNA

Brigitte Bruijns; Roald Tiggelaar; Han Gardeniers

doi:10.1016/j.dib.2016.11.090

Dataset of the absorption, emission and excitation spectra and fluorescence intensity graphs of fluorescent cyanine dyes for the quantification of low amounts of dsDNA

Data Brief. 2016 Nov 28:10:132-143. doi: 10.1016/j.dib.2016.11.090. eCollection 2017 Feb.

Authors

Brigitte Bruijns¹, Roald Tiggelaar², Han Gardeniers²

Affiliations

¹ Mesoscale Chemical Systems, MESA Institute for Nanotechnology, University of Twente, Enschede, The Netherlands; Life Science, Life Science, Engineering & Design, Saxion University of Applied Sciences, Enschede, The Netherlands.
² Mesoscale Chemical Systems, MESA Institute for Nanotechnology, University of Twente, Enschede, The Netherlands.

Abstract

This article describes data related to a research article entitled "Fluorescent cyanine dyes for the quantification of low amounts of dsDNA" (B. Bruijns, R. Tiggelaar, J. Gardeniers, 2016) [1]. Six cyanine dsDNA dyes - EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1 - are investigated and in this article the absorption spectra, as well as excitation and emission spectra, for all six researched cyanine dyes are given, all recorded under exactly identical experimental conditions. The intensity graphs, with the relative fluorescence in the presence of low amounts of dsDNA, are also provided.