High-throughput sequencing analysis reveals the genetic diversity of different regions of the murine norovirus genome during in vitro replication

Arch Virol. 2017 Apr;162(4):1019-1023. doi: 10.1007/s00705-016-3179-y. Epub 2016 Dec 9.

Abstract

In this study, we report the genetic diversity and nucleotide mutation rates of five representative regions of the murine norovirus genome during in vitro passages. The mutation rates were similar in genomic regions encompassing partial coding sequences for non-structural (NS) 1-2, NS5, NS6, NS7 proteins within open reading frame (ORF) 1. In a region encoding a portion of the major capsid protein (VP1) within ORF2 (also including the ORF4 region) and a portion of the minor structural protein (VP2), the mutation rates were estimated to be at least one order of magnitude higher. The VP2 coding region was found to have the highest mutation rate.

Keywords: Genetic diversity; Murine; Mutation; Next-generation sequencing; Norovirus.

MeSH terms

  • Animals
  • Caliciviridae Infections / veterinary*
  • Caliciviridae Infections / virology
  • DNA Replication
  • Genetic Variation*
  • Genome, Viral*
  • High-Throughput Nucleotide Sequencing
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Norovirus / genetics*
  • Norovirus / isolation & purification
  • Norovirus / physiology
  • Open Reading Frames
  • Rodent Diseases / virology*
  • Serial Passage
  • Viral Nonstructural Proteins / genetics*
  • Viral Nonstructural Proteins / metabolism
  • Virus Replication

Substances

  • Viral Nonstructural Proteins