Abstract
A phytochemical investigation to obtain new NO inhibitors resulted in the identification of six new (1-6) and four known (7-10) terpenoids from Salvia plebeia. Compounds 1 and 2 are new diterpenoids, 3-5 are new meroditerpenoids, 6-9 are sesquiterpenoids, and 10 is a known meroditerpenoid. The structures of these isolates were determined by routine NMR experiments and X-ray diffraction, as well as the electronic circular dichroism spectra. Compounds 1-4 are diterpenoids carrying an oxygen bridge, and 6 is a rare copane-type sesquiterpenoid with a bridged tricyclic framework. The isolates inhibited NO generation induced by lipopolysaccharide in BV-2 cells. The possible mechanism of NO inhibition of some bioactive compounds was also investigated using molecular docking, which revealed interactions of bioactive compounds with the iNOS protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Crystallography, X-Ray
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Diterpenes / chemistry
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Diterpenes / isolation & purification*
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Diterpenes / pharmacology*
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / isolation & purification*
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Drugs, Chinese Herbal / pharmacology*
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Molecular Conformation
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Molecular Docking Simulation
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Molecular Structure
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Nitric Oxide / antagonists & inhibitors*
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Nitric Oxide / biosynthesis
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Nitric Oxide Synthase Type II / metabolism*
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Nuclear Magnetic Resonance, Biomolecular
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Salvia / chemistry*
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Sesquiterpenes / chemistry
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Sesquiterpenes / isolation & purification
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Sesquiterpenes / pharmacology
Substances
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Diterpenes
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Drugs, Chinese Herbal
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Lipopolysaccharides
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Sesquiterpenes
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Nitric Oxide
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NOS2 protein, human
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Nitric Oxide Synthase Type II