NMR-Based Metabolomics of Oral Biofluids

Horst Joachim Schirra; Pauline J Ford

doi:10.1007/978-1-4939-6685-1_5

NMR-Based Metabolomics of Oral Biofluids

Methods Mol Biol. 2017:1537:79-105. doi: 10.1007/978-1-4939-6685-1_5.

Authors

Horst Joachim Schirra¹, Pauline J Ford²

Affiliations

¹ Centre for Advanced Imaging, The University of Queensland, Brisbane, QLD, 4072, Australia. h.schirra@uq.edu.au.
² School of Dentistry, Oral Health Centre, The University of Queensland, 288 Herston Road, Herston, QLD, 4006, Australia.

PMID: 27924589
DOI: 10.1007/978-1-4939-6685-1_5

Abstract

NMR-based metabolomics is an established technique for characterizing the metabolite profile of biological fluids and investigating how metabolite profiles change in response to biological and/or clinical stimuli. Thus, NMR-based metabolomics has the potential to discover biomarkers for diagnosis, prognosis, and/or therapy of clinical conditions, as well as to unravel the physiology underlying clinical conditions. Here, we describe a detailed protocol for NMR-based metabolomics of oral biofluids, including sample collection, sample handling, NMR data acquisition, and processing. In addition, we give a general overview of the statistical analysis of the resulting metabolomic data.

Keywords: Gingival crevicular fluid; Metabolomics; NMR spectroscopy; Saliva; Systems biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Computational Biology / methods
Data Interpretation, Statistical
Gingival Crevicular Fluid / metabolism*
Humans
Magnetic Resonance Spectroscopy / methods*
Metabolomics / methods*
Proteome*
Saliva / metabolism*

Substances

Proteome