The effects of a multi-ingredient supplement on markers of muscle damage and inflammation following downhill running in females

Jessica L Köhne; Michael J Ormsbee; Andrew J McKune

doi:10.1186/s12970-016-0156-5

The effects of a multi-ingredient supplement on markers of muscle damage and inflammation following downhill running in females

J Int Soc Sports Nutr. 2016 Nov 25:13:44. doi: 10.1186/s12970-016-0156-5. eCollection 2016.

Authors

Jessica L Köhne¹, Michael J Ormsbee², Andrew J McKune³

Affiliations

¹ Discipline of Biokinetics, Exercise and Leisure Sciences, School of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
² Discipline of Biokinetics, Exercise and Leisure Sciences, School of Health Sciences, University of KwaZulu-Natal, Durban, South Africa ; Department of Nutrition, Food and Exercise Sciences, Institute of Sport Sciences and Medicine, Florida State University, Tallahassee, USA.
³ Discipline of Biokinetics, Exercise and Leisure Sciences, School of Health Sciences, University of KwaZulu-Natal, Durban, South Africa ; Department of Nutrition, Food and Exercise Sciences, Institute of Sport Sciences and Medicine, Florida State University, Tallahassee, USA ; Discipline of Sport and Exercise Science, University of Canberra Research Institute for Sport and Exercise, Faculty of Health, University of Canberra, Canberra, Australia.

Abstract

Background: The effects of a multi-ingredient performance supplement (MIPS) on markers of inflammation and muscle damage, perceived soreness and lower limb performance are unknown in endurance-trained female athletes. The purpose of this study was to determine the impact of MIPS (NO-Shotgun®) pre-loaded 4 weeks prior to a single-bout of downhill running (DHR) on hsC-Reactive Protein (hsCRP), interleukin (IL)-6, creatine kinase (CK), muscle soreness, lower limb circumferences and performance.

Method: Trained female runners (n = 8; 29 ± 5.9 years) (VO_2max: ≥ 50 ml^-1.kg^-1.min^-1, midfollicular phase (7-11 days post-menses) were randomly assigned in a double-blind manner into two groups: MIPS (n = 4) ingested one serving of NO Shotgun daily for 28 days prior to DHR and 30 min prior to all post-testing visits; Control (CON) (n = 4) consumed an isocaloric maltodextrin placebo in an identical manner to MIPS. hsCRP, IL-6, CK, perceived soreness, limb circumferences, and performance measures (flexibility, squat jump peak power) were tested on 5 occasions; immediately before (PRE), immediately post-DHR, 24, 48 and 72 h post-DHR.

Results: There were main effects of time for CK (p = 0.05), pain pressure threshold (right tibialis anterior (p = 0.010), right biceps femoris (p = 0.01), and left iliotibial band (ITB) (p = 0.05) across all time points), and maximum squat jump power (p = 0.04). Compared with 24 h post-DHR, maximum squat jump power was significantly lower at 48 h post-DHR (p = 0.05). Lower body perceived soreness was significantly increased at 24 h (p = 0.02) and baseline to 48 h (p = 0.02) post DHR. IL-6 peaked immediately post-DHR (p = 0.03) and hsCRP peaked at 24 h post-DHR (p = 0.06). Calculation of effect sizes indicated a moderate attenuation of hsCRP in MIPS at 72 h post-DHR.

Conclusions: Consumption of MIPS for 4 weeks prior to a single bout of DHR attenuated inflammation three days post, but did not affect perceived soreness and muscle damage markers in endurance trained female runners following a single bout of DHR.

Keywords: Eccentric exercise; Muscle soreness; Recovery.

Publication types

Randomized Controlled Trial

MeSH terms

Adult
Athletes*
Biomarkers / blood
Creatine Kinase / blood
Dietary Supplements*
Double-Blind Method
Female
Humans
Inflammation / blood*
Interleukin-6 / blood
Myalgia / blood*
Running / physiology*
Sports Nutritional Physiological Phenomena / drug effects

Substances

Biomarkers
IL6 protein, human
Interleukin-6
Creatine Kinase