Abstract
Iron-chelating activity of synthesized spirocyclic hydroxamic acids, their toxicity, and effects on mitochondrial function were studied using primary culture of cerebral cortical neurons from newborn rats. All tested compounds effectively chelated Fe(II) ions. Activity of spirocyclic hydroxamic acids more strictly depended on the structure their piperidine, but not imidazolidine fragment. All compounds were non-toxic for normal neuronal culture.
Keywords:
chelators; hydroxamic acids; mitochondria; toxicity.
MeSH terms
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Animals
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Animals, Newborn
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Animals, Outbred Strains
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Cations, Divalent
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Cell Survival / drug effects
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Cerebral Cortex / cytology
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Cerebral Cortex / drug effects
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Cerebral Cortex / metabolism
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Ferrozine / chemistry
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Hydroxamic Acids / chemical synthesis
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Hydroxamic Acids / pharmacology*
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Iron / metabolism*
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Iron Chelating Agents / chemical synthesis
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Iron Chelating Agents / pharmacology*
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Male
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Membrane Potential, Mitochondrial / drug effects
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Mitochondria, Liver / drug effects*
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Mitochondria, Liver / metabolism
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Mitochondrial Swelling / drug effects
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Neurons / drug effects*
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Neurons / metabolism
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Primary Cell Culture
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Rats
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Spiro Compounds / chemical synthesis
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Spiro Compounds / pharmacology*
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Structure-Activity Relationship
Substances
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Cations, Divalent
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Hydroxamic Acids
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Iron Chelating Agents
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Spiro Compounds
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Ferrozine
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Iron