Methods for Proteome Analysis of Campylobacter jejuni Using 2-D Electrophoresis

Ramila C Rodrigues; Nabila Haddad; Odile Tresse

doi:10.1007/978-1-4939-6536-6_19

Methods for Proteome Analysis of Campylobacter jejuni Using 2-D Electrophoresis

Methods Mol Biol. 2017:1512:233-244. doi: 10.1007/978-1-4939-6536-6_19.

Authors

Ramila C Rodrigues^{1

2}, Nabila Haddad^{1

2}, Odile Tresse^{3

4}

Affiliations

¹ INRA, UMR 1014 Secalim, Nantes, F-44307, France.
² LUNAM Université Oniris, Nantes, F-44307, France.
³ INRA, UMR 1014 Secalim, Nantes, F-44307, France. odile.tresse@oniris-nantes.fr.
⁴ LUNAM Université Oniris, Nantes, F-44307, France. odile.tresse@oniris-nantes.fr.

PMID: 27885611
DOI: 10.1007/978-1-4939-6536-6_19

Abstract

This chapter describes protocols used for two-dimensional electrophoretic analysis of the proteome or subproteome of Campylobacter jejuni, a major human food-borne pathogen. The following protocols, adapted to Campylobacter strains, include all the steps from cultivation to gel-support protein separation.

Keywords: 2-D electrophoresis; Campylobacter; Isoelectric point; Protein extraction; Protein purification; SDS-PAGE.

MeSH terms

Bacterial Proteins / classification
Bacterial Proteins / isolation & purification*
Bacterial Proteins / metabolism
Campylobacter jejuni / chemistry*
Campylobacter jejuni / growth & development
Campylobacter jejuni / metabolism
Culture Media / chemistry
Densitometry
Dialysis
Electrophoresis, Gel, Two-Dimensional / instrumentation
Electrophoresis, Gel, Two-Dimensional / methods*
Electrophoresis, Polyacrylamide Gel / methods*
Isoelectric Focusing / methods*
Isoelectric Point
Proteome / isolation & purification*
Proteome / metabolism
Silver Staining

Substances

Bacterial Proteins
Culture Media
Proteome