Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix

Meiqing Guo; Hefeng Wang; Di Huang; Zhijun Han; Qiang Li; Xiaojun Wang; Jing Chen

doi:10.1088/1468-6996/15/3/035005

Amperometric catechol biosensor based on laccase immobilized on nitrogen-doped ordered mesoporous carbon (N-OMC)/PVA matrix

Sci Technol Adv Mater. 2014 Jun 10;15(3):035005. doi: 10.1088/1468-6996/15/3/035005. eCollection 2014 Jun.

Authors

Meiqing Guo¹, Hefeng Wang², Di Huang², Zhijun Han², Qiang Li³, Xiaojun Wang², Jing Chen⁴

Affiliations

¹ College of Mechanics, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China; Shanxi Key Laboratory of Material Strength and Structural Impact, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China; Authors to whom any correspondence should be addressed.
² College of Mechanics, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China; Shanxi Key Laboratory of Material Strength and Structural Impact, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China.
³ Shanxi Coal Bed Methane Co., Ltd , Taiyuan 030024, People's Republic of China.
⁴ Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology ,Taiyuan 030024, People's Republic of China; Shanxi Key Laboratory of Material Strength and Structural Impact, Taiyuan University of Technology , Taiyuan 030024, People's Republic of China; Authors to whom any correspondence should be addressed.

Abstract

A functionalized nitrogen-containing ordered mesoporous carbon (N-OMC), which shows good electrical properties, was synthesized by the carbonization of polyaniline inside a SBA-15 mesoporous silica template. Based on this, through entrapping laccase onto the N-OMC/polyvinyl alcohol (PVA) film a facilely fabricated amperometric biosensor was developed. Laccase from Trametes versicolor was assembled on a composite film of a N-OMC/PVA modified Au electrode and the electrochemical behavior was investigated. The results indicated that the N-OMC modified electrode exhibits electrical properties towards catechol. The optimum experimental conditions of a biosensor for the detection of catechol were studied in detail. Under the optimal conditions, the sensitivity of the biosensor was 0.29 A*M^-1 with a detection limit of 0.31 μM and a linear detection range from 0.39 μM to 8.98 μM for catechol. The calibration curve followed the Michaelis-Menten kinetics and the apparent Michaelis-Menten [Formula: see text] was 6.28 μM. This work demonstrated that the N-OMC/PVA composite provides a suitable support for laccase immobilization and the construction of a biosensor.

Keywords: N-OMC; PVA; biosensor; electrical properties; laccase.