Establishment of EBV-Infected Lymphoblastoid Cell Lines

Noemi Nagy

doi:10.1007/978-1-4939-6655-4_3

Establishment of EBV-Infected Lymphoblastoid Cell Lines

Methods Mol Biol. 2017:1532:57-64. doi: 10.1007/978-1-4939-6655-4_3.

Author

Noemi Nagy¹

Affiliation

¹ Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Nobels väg 16, SE-171 77, Stockholm, Sweden. noemi.nagy@ki.se.

PMID: 27873266
DOI: 10.1007/978-1-4939-6655-4_3

Abstract

Lymphoblastoid cell lines (LCLs) can be generated easily by in vitro EBV infection of B lymphocytes collected from any individual. In vitro, these EBV-infected B cell cultures yield proliferating, transformed lines referred to as lymphoblastoid cell lines (LCLs).

Keywords: Akata; B95-8; Cyclosporin A; LCL; Mononuclear cells.

MeSH terms

B-Lymphocytes / metabolism
B-Lymphocytes / pathology
B-Lymphocytes / virology*
Cell Line, Transformed
Cell Separation / methods
Cell Transformation, Viral*
Epstein-Barr Virus Infections / virology*
Herpesvirus 4, Human / physiology*
Humans
Leukocytes, Mononuclear / metabolism
Leukocytes, Mononuclear / virology
Transduction, Genetic
Virus Replication