Small molecule absorption by PDMS in the context of drug response bioassays

B J van Meer; H de Vries; K S A Firth; J van Weerd; L G J Tertoolen; H B J Karperien; P Jonkheijm; C Denning; A P IJzerman; C L Mummery

doi:10.1016/j.bbrc.2016.11.062

Small molecule absorption by PDMS in the context of drug response bioassays

Biochem Biophys Res Commun. 2017 Jan 8;482(2):323-328. doi: 10.1016/j.bbrc.2016.11.062. Epub 2016 Nov 14.

Authors

B J van Meer¹, H de Vries², K S A Firth³, J van Weerd⁴, L G J Tertoolen⁵, H B J Karperien⁶, P Jonkheijm⁷, C Denning⁸, A P IJzerman⁹, C L Mummery¹⁰

Affiliations

¹ Dept. of Anatomy and Embryology, Leiden University Medical Centre, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands. Electronic address: b.j.vanmeer@lumc.nl.
² Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333 CC, Leiden, The Netherlands. Electronic address: vries_h@lacdr.leidenuniv.nl.
³ Dept. of Stem Cell Biology, University of Nottingham, University Park, Nottingham, NG7 2RD, UK. Electronic address: mzxksf@nottingham.ac.uk.
⁴ LipoCoat B.V., PO Box 217, 7500 AE, Enschede, The Netherlands. Electronic address: j.vanweerd@utwente.nl.
⁵ Dept. of Anatomy and Embryology, Leiden University Medical Centre, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands. Electronic address: l.g.j.tertoolen@lumc.nl.
⁶ LipoCoat B.V., PO Box 217, 7500 AE, Enschede, The Netherlands; Dept. of Developmental BioEngineering, University of Twente, Driernerlolaan 5, 7522 NB, Enschede, The Netherlands. Electronic address: h.b.j.karperien@utwente.nl.
⁷ LipoCoat B.V., PO Box 217, 7500 AE, Enschede, The Netherlands; Dept. of Molecular Nanofabrication, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands. Electronic address: p.jonkheijm@utwente.nl.
⁸ Dept. of Stem Cell Biology, University of Nottingham, University Park, Nottingham, NG7 2RD, UK. Electronic address: Chris.Denning@nottingham.ac.uk.
⁹ Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333 CC, Leiden, The Netherlands. Electronic address: ijzerman@lacdr.leidenuniv.nl.
¹⁰ Dept. of Anatomy and Embryology, Leiden University Medical Centre, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands; Dept. of Applied Stem Cell Technologies, University of Twente, P.O. Box 217, 7500 AE, Enschede, The Netherlands. Electronic address: c.l.mummery@lumc.nl.

Abstract

The polymer polydimethylsiloxane (PDMS) is widely used to build microfluidic devices compatible with cell culture. Whilst convenient in manufacture, PDMS has the disadvantage that it can absorb small molecules such as drugs. In microfluidic devices like "Organs-on-Chip", designed to examine cell behavior and test the effects of drugs, this might impact drug bioavailability. Here we developed an assay to compare the absorption of a test set of four cardiac drugs by PDMS based on measuring the residual non-absorbed compound by High Pressure Liquid Chromatography (HPLC). We showed that absorption was variable and time dependent and not determined exclusively by hydrophobicity as claimed previously. We demonstrated that two commercially available lipophilic coatings and the presence of cells affected absorption. The use of lipophilic coatings may be useful in preventing small molecule absorption by PDMS.

Keywords: Absorption; Drug screening; LipoCoat Cellbinder; Microfluidics; PDMS; PDMS coating.

MeSH terms

Absorption, Physicochemical
Biological Assay / methods*
Cardiovascular Agents / chemistry*
Cardiovascular Agents / isolation & purification
Chromatography, High Pressure Liquid / instrumentation*
Chromatography, High Pressure Liquid / methods
Coated Materials, Biocompatible / chemistry
Dimethylpolysiloxanes / chemistry*
Drug Evaluation, Preclinical / methods*
Equipment Design
Equipment Failure Analysis
Lab-On-A-Chip Devices*
Lipids / chemistry
Materials Testing
Nylons / chemistry*
Pharmaceutical Preparations

Substances

Cardiovascular Agents
Coated Materials, Biocompatible
Dimethylpolysiloxanes
Lipids
Nylons
Pharmaceutical Preparations
poly(dimethylsiloxane)-polyamide copolymer

Abstract

MeSH terms

Substances

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