Fabrication and characterization of graphene-based hybrid nanocomposite: assessment of antibacterial potential and biomedical application

Artif Cells Nanomed Biotechnol. 2017 Dec;45(8):1496-1508. doi: 10.1080/21691401.2016.1252384. Epub 2016 Nov 16.

Abstract

The present investigation deals with synthesis of graphene oxide (GO) and fabrication of GO-based hybrid nanocomposites (Ncs). Synthesized GO and Ncs were primarily confirmed by UV visible and Fourier transform infrared (FT-IR) spectroscopy. Fabricated Ncs showed potential antimicrobial activity against Gram-positive and Gram-negative bacterial strains. Surface morphology, Elemental analysis, and FTIR imaging analysis were carried out to confirm Ncs formation. The Ncs were impregnated into the pullulan polymeric layer-by-layer (LbL) ultrathin film by using novel spin-coating approach. Mechanical properties were determined using Brookfield texture analyzer, and percentage moisture content confirmed the physicochemical stability of LbL film.

Keywords: Graphene; antimicrobial; hybrid nanocomposite; layer-by-layer film; spin coating.

MeSH terms

  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacology*
  • Anti-Bacterial Agents / toxicity
  • Chitosan / chemistry
  • Graphite / chemistry*
  • HeLa Cells
  • Humans
  • Nanocomposites / chemistry*
  • Nanocomposites / toxicity
  • Oxides / chemistry
  • Particle Size
  • Tensile Strength
  • Water / chemistry

Substances

  • Anti-Bacterial Agents
  • Oxides
  • Water
  • Graphite
  • Chitosan