Comparison of monoplex and duplex RT-PCR assays for the detection of measles virus

J Virol Methods. 2017 Jan:239:58-60. doi: 10.1016/j.jviromet.2016.11.003. Epub 2016 Nov 9.

Abstract

Rapid and accurate detection of measles virus is important for case diagnosis and public health management. This study compared the performance of two monoplex RT-PCR reactions targeting the H and N genes to a duplex RT-PCR targeting both genes simultaneously. The duplex simplified processing without compromising assay performance characteristic.

Keywords: Canada; Hemagglutinin; Measles; Method; Molecular; Neuraminidase; PCR.

Publication types

  • Comparative Study

MeSH terms

  • Canada / epidemiology
  • DNA Primers
  • Hemagglutinins, Viral / genetics
  • Humans
  • Measles / diagnosis*
  • Measles / epidemiology
  • Measles / virology
  • Measles virus / genetics
  • Measles virus / isolation & purification*
  • Molecular Diagnostic Techniques*
  • Multiplex Polymerase Chain Reaction*
  • Neuraminidase / genetics
  • Public Health
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction*
  • Sensitivity and Specificity

Substances

  • DNA Primers
  • Hemagglutinins, Viral
  • RNA, Viral
  • Neuraminidase