Draft Genome Sequences of Two Ralstonia pickettii Strains with Different Aminoglycoside Resistance Phenotypes

Ivone Vaz-Moreira; Javier Tamames; José Luis Martínez; Célia M Manaia

doi:10.1128/genomeA.01257-16

Draft Genome Sequences of Two Ralstonia pickettii Strains with Different Aminoglycoside Resistance Phenotypes

Genome Announc. 2016 Nov 10;4(6):e01257-16. doi: 10.1128/genomeA.01257-16.

Authors

Ivone Vaz-Moreira^{1

2}, Javier Tamames³, José Luis Martínez⁴, Célia M Manaia⁵

Affiliations

¹ Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal.
² LEPABE-Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia, Faculdade de Engenharia, Universidade do Porto, Porto, Portugal.
³ Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Cantoblanco, Madrid, Spain jtamames@cnb.csis.es cmanaia@porto.ucp.pt.
⁴ Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Cantoblanco, Madrid, Spain.
⁵ Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina, Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal jtamames@cnb.csis.es cmanaia@porto.ucp.pt.

Abstract

The genomes of two Ralstonia pickettii strains (H2Cu2 and H2Cu5), isolated from hospital effluent in a selective medium containing CuSO_4, were sequenced. They presented MICs of >256 and 6 µg/ml for the aminoglycoside gentamicin, respectively. The 5.2-Mb draft genomes have 40 contigs for strain H2Cu2 and 113 for H2Cu5.

Grants and funding

This research was partially funded by the Fundação para a Ciência e a Tecnologia (FCT) through projects UID/Multi/50016/2013 and NanoDiaBac ENMed/0001/2014 and by FCT and MINECO through the project WaterJPI/0001/2013 STARE (Stopping Antibiotic Resistance Evolution). I.V.-M. was supported by FCT grant SFRH/BPD/87360/2012, and international cooperation was funded by COST-European Cooperation in Science and Technology under COST Action ES1403: New and Emerging Challenges and Opportunities in Wastewater Reuse (NEREUS).