MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells

Irene K Oglesby; Paul J McKiernan

doi:10.1007/978-1-4939-6524-3_7

MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells

Methods Mol Biol. 2017:1509:57-69. doi: 10.1007/978-1-4939-6524-3_7.

Authors

Irene K Oglesby¹, Paul J McKiernan²

Affiliations

¹ Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin, Ireland. ioglesby@rcsi.ie.
² Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin, Ireland.

PMID: 27826918
DOI: 10.1007/978-1-4939-6524-3_7

Abstract

Bronchial epithelial cells represent an invaluable tool to elucidate molecular signaling regulation in cystic fibrosis (CF). CF is a lethal genetic condition characterized by chronic inflammation in which bronchial epithelial cells play a pivotal role. Here we describe their use in analysis of microRNA (miRNA) and their target genes following a two-step RT-PCR miRNA profiling method in bronchial cell specimens from CF and control individuals where 667 human miRNA were examined. We also describe an approach to experimental modulation of these miRNA in vitro.

Keywords: Array; Bronchial cells; Cystic fibrosis; Transfection; miRNA; qRT-PCR.

MeSH terms

Bronchi / metabolism
Bronchi / pathology
Cell Culture Techniques
Cell Line
Cystic Fibrosis / metabolism*
Cystic Fibrosis / pathology
Epithelial Cells / metabolism
Gene Expression Profiling*
Humans
MicroRNAs / genetics*
MicroRNAs / isolation & purification
MicroRNAs / metabolism
Real-Time Polymerase Chain Reaction
Respiratory Mucosa / metabolism
Respiratory Mucosa / pathology
Transcriptome

Substances

MicroRNAs