Investigation of the effects of GABA receptor agonists in the differentiation of human induced pluripotent stem cells into dopaminergic neurons

Dokl Biol Sci. 2016 Sep;470(1):244-246. doi: 10.1134/S0012496616050045. Epub 2016 Nov 8.

Abstract

The influence of GABA receptor agonists on the terminal differentiation in vitro of dopaminergic (DA) neurons derived from IPS cells was investigated. GABA-A agonist muscimol induced transient elevation of intracellular Ca2+ level ([Ca2+] i ) in the investigated cells at days 5 to 21 of differentiation. Differentiation of cells in the presence of muscimol reduced tyrosine hydroxylase expression. Thus, the presence of active GABA-A receptors, associated with phenotype determination via Ca2+-signalling was demonstrated in differentiating human DA neurons.

MeSH terms

  • Baclofen / administration & dosage
  • Calcium / metabolism
  • Calcium Signaling / drug effects
  • Calcium Signaling / physiology
  • Cell Differentiation / drug effects
  • Cell Differentiation / physiology
  • Cells, Cultured
  • Dopaminergic Neurons / cytology*
  • Dopaminergic Neurons / drug effects
  • Dopaminergic Neurons / metabolism*
  • GABA Agonists / administration & dosage*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / drug effects
  • Induced Pluripotent Stem Cells / metabolism*
  • Muscimol / administration & dosage
  • Receptors, GABA-A / metabolism*

Substances

  • GABA Agonists
  • Receptors, GABA-A
  • Muscimol
  • Baclofen
  • Calcium