A MVA construct expressing a secretable form of the Dengue virus 3 envelope protein protects immunized mice from dengue-induced encephalitis

Vaccine. 2016 Dec 7;34(50):6120-6122. doi: 10.1016/j.vaccine.2016.10.058. Epub 2016 Nov 3.

Abstract

Dengue is no longer restricted to tropical developing countries, but is now a major global public health problem. Despite the recent license approval of the CYD-TDV vaccine in some countries, efforts to develop a more efficient vaccine against Dengue virus (DENV) continue. Herein, we evaluate the immunogenicity and level of protection of two potential vaccines against DENV based on recombinant modified vaccinia virus Ankara (rMVA). The vaccine addressing the Envelope protein from DENV serotype 3 to the endoplasmic reticulum elicited neutralizing antibodies titers which correlate with protection, and also confers protection upon challenge in a mouse model. Our results support the development of a tetravalent dengue vaccine with the further construction of rMVAs expressing proteins from the other DENV serotypes.

Keywords: Dengue vaccine; Encephalitis model; MVA; Neutralizing antibodies; Recombinant MVA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Neutralizing / blood
  • Antibodies, Viral / blood
  • Dengue / prevention & control*
  • Dengue Vaccines / administration & dosage
  • Dengue Vaccines / genetics
  • Dengue Vaccines / immunology*
  • Dengue Virus / genetics
  • Dengue Virus / immunology*
  • Disease Models, Animal
  • Drug Carriers*
  • Encephalitis, Viral / prevention & control*
  • Female
  • Mice
  • Mice, Inbred C57BL
  • Vaccinia virus / genetics*
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology*

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Dengue Vaccines
  • Drug Carriers
  • Viral Envelope Proteins