The assembly line is one of the key features of industrial production on the macroscopic scale, allowing programmability and sequential addition of parts to a final product. In this chapter, we use DNA to extend this notion to the nanoscale by the judicious combination of three DNA-based components: a DNA origami tile that provides a framework and track for the assembly process, three two-state DNA cassettes that can be programmed to donate cargo and are attached to the tile, and a DNA walker that can move on the track to collect cargo.
Keywords: Assembly line; DNA cassettes; DNA origami; DNA walker.