Biological conversion of the aqueous wastes from hydrothermal liquefaction of algae and pine wood by Rhodococci

Yucai He; Xiaolu Li; Xiaoyun Xue; Marie S Swita; Andrew J Schmidt; Bin Yang

doi:10.1016/j.biortech.2016.10.059

Biological conversion of the aqueous wastes from hydrothermal liquefaction of algae and pine wood by Rhodococci

Bioresour Technol. 2017 Jan:224:457-464. doi: 10.1016/j.biortech.2016.10.059. Epub 2016 Oct 21.

Authors

Yucai He¹, Xiaolu Li¹, Xiaoyun Xue¹, Marie S Swita², Andrew J Schmidt², Bin Yang³

Affiliations

¹ Bioproducts, Sciences and Engineering Laboratory and Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, United States.
² Energy and Efficiency Division and the Bioproduct Sciences and Engineering Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354, United States.
³ Bioproducts, Sciences and Engineering Laboratory and Department of Biological Systems Engineering, Washington State University, Richland, WA 99354, United States. Electronic address: binyang@tricity.wsu.edu.

PMID: 27806887
DOI: 10.1016/j.biortech.2016.10.059

Abstract

In this study, R. opacus PD630, R. jostii RHA1, R. jostii RHA1 VanA^-, and their co-culture were employed to convert hydrothermal liquefaction aqueous waste (HTLAW) into lipids. After 11days, the COD reduction of algal-HTLAW reached 93.4% and 92.7% by R. jostii RHA1 and its mutant VanA^-, respectively. Woody-HTLAW promoted lipid accumulation of 0.43glipid/gcell dry weight in R. opacus PD630 cells. Additionally, the total number of chemicals in HTLAW decreased by over 1/3 after 7days of coculture, and 0.10g/L and 0.46g/L lipids were incrementally accumulated in the cellular mass during the fermentation of wood- and algal-HTLAW, respectively. The GC-MS data supported that different metabolism pathways were followed when these Rhodococci strains degraded algae- and woody-HTLAW. These results indicated promising potential of bioconversion of under-utilized carbon and toxic compounds in HTLAW into useful products by selected Rhodococci.

Keywords: Bioconversion; Coculture; Hydrothermal liquefaction aqueous waste; Lipid; Oleaginous Rhodococci.

MeSH terms

Carbon / metabolism
Chlorophyta / metabolism*
Fermentation
Gas Chromatography-Mass Spectrometry
Lipids
Pinus / metabolism
Rhodococcus / metabolism*
Waste Management / methods
Water / metabolism
Wood / metabolism*

Substances

Lipids
Water
Carbon