Decreased paraoxonase1 activity and increased malondialdehyde and oxidative DNA damage levels in primary open angle glaucoma

Ugur Yilmaz Mumcu; Ibrahim Kocer; Orhan Ates; H Hakan Alp

doi:10.18240/ijo.2016.10.24

Decreased paraoxonase1 activity and increased malondialdehyde and oxidative DNA damage levels in primary open angle glaucoma

Int J Ophthalmol. 2016 Oct 18;9(10):1518-1520. doi: 10.18240/ijo.2016.10.24. eCollection 2016.

Authors

Ugur Yilmaz Mumcu¹, Ibrahim Kocer², Orhan Ates², H Hakan Alp³

Affiliations

¹ Department of Ophthalmology, Haydarpasa Training and Research Hospital, Istanbul 34668, Turkey.
² Department of Ophthalmology, Faculty of Medicine, Atatürk University, Erzurum 25040, Turkey.
³ Department of Biochemistry, Atatürk University, Erzurum 25040, Turkey.

Abstract

To investigate the malondialdehyde (MDA) levels, paraoxonase1 (PON1) activity and 8-hydroxy 2-deoxyguanosine (8-OHdG) levels in the primary open angle glaucoma (POAG) patient. Blood samples from 52 healthy individuals and 53 patients with POAG were analyzed for MDA and 8-OHdG by HPLC (high-performance liquid chromatography) and PON1 by spectrophotometry. The data obtained were analyzed statistically. MDA levels were 10.46±8.4 and 4.70±1.79 µmol; PON1 levels were 121±39.55 and 161.62±60.22 U/mL; and 8-OHdG values were 1.32±0.53/10⁶ dG and 0.47±0.27/10⁶ dG in the POAG patients and the control group, respectively. The difference was significant in MDA levels, 8-OHdG levels and PON1 activity in POAG patients in comparison with controls (P<0.001). We concluded that the observed increase in MDA and 8-OHdG levels may be correlated with decreased PON1 activity. Oxidative stress plays an important role in glaucoma development.

Keywords: 8-hydroxy 2-deoxyguanosine; glaucoma; paraoxonase.