Routine identification of Nocardia species by MALDI-TOF mass spectrometry

Victoria Girard; Sandrine Mailler; Sophie Polsinelli; Delphine Jacob; Marie Christine Saccomani; Beatrice Celliere; Valerie Monnin; Alex van Belkum; Ferry Hagen; Jacques F Meis; Géraldine Durand

doi:10.1016/j.diagmicrobio.2016.09.024

Routine identification of Nocardia species by MALDI-TOF mass spectrometry

Diagn Microbiol Infect Dis. 2017 Jan;87(1):7-10. doi: 10.1016/j.diagmicrobio.2016.09.024. Epub 2016 Oct 4.

Affiliations

¹ R&D Microbiology, bioMérieux, La Balme Les Grottes, France. Electronic address: victoria.girard@biomerieux.com.
² R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
³ Canisius-Wilhelmina Hospital, Department of Medical Microbiology and Infectious Diseases, Nijmegen, The Netherlands.
⁴ Canisius-Wilhelmina Hospital, Department of Medical Microbiology and Infectious Diseases, Nijmegen, The Netherlands; Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.

PMID: 27802877
DOI: 10.1016/j.diagmicrobio.2016.09.024

Abstract

We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3.0).

Keywords: MALDI TOF MS; Nocardia; Routine diagnostic microbiology; Species identification.

MeSH terms

Bacteriological Techniques / methods*
Humans
Nocardia / chemistry*
Nocardia / classification*
Nocardia Infections / diagnosis*
Specimen Handling / methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*