Complete Genome Sequence of an Ebola Virus Isolate Imported from Sierra Leone to Germany Determined by Circle Sequencing

Jan Philipp Mengel; Artur Lissin; Nadine Biedenkopf; Tilman Schultze; Gopala Krishna Mannala; Gordian Schudt; Gerrit Kann; Timo Wolf; Markus Eickmann; Stephan Becker; Franz Cemic; Torsten Hain

doi:10.1128/genomeA.01011-16

Complete Genome Sequence of an Ebola Virus Isolate Imported from Sierra Leone to Germany Determined by Circle Sequencing

Genome Announc. 2016 Oct 6;4(5):e01011-16. doi: 10.1128/genomeA.01011-16.

Authors

Jan Philipp Mengel¹, Artur Lissin², Nadine Biedenkopf^{3

4}, Tilman Schultze¹, Gopala Krishna Mannala¹, Gordian Schudt^{3

4}, Gerrit Kann⁵, Timo Wolf⁵, Markus Eickmann^{3

4}, Stephan Becker^{3

4}, Franz Cemic⁶, Torsten Hain^{7

4}

Affiliations

¹ Institute for Medical Microbiology, Justus-Liebig University of Giessen, Giessen, Germany.
² Institute for Biochemical Engineering and Analytics, University of Applied Sciences, Giessen, Germany.
³ Institute of Virology, Philipps University of Marburg, Marburg, Germany.
⁴ German Center for Infection Research (DZIF), partner site Giessen-Marburg-Langen, Germany.
⁵ Department of Medicine, Infectious Diseases Unit, University Hospital Frankfurt, Frankfurt am Main, Germany.
⁶ Institute for Biochemical Engineering and Analytics, University of Applied Sciences, Giessen, Germany franz.cemic@mni.thm.de torsten.hain@mikrobio.med.uni-giessen.de.
⁷ Institute for Medical Microbiology, Justus-Liebig University of Giessen, Giessen, Germany franz.cemic@mni.thm.de torsten.hain@mikrobio.med.uni-giessen.de.

Abstract

We report here a complete genome sequence of Ebola virus Makona from a nonfatal patient sample that originated in Sierra Leone during the last Ebola virus outbreak in West Africa (species Zaire ebolavirus) using a highly accurate circle sequencing (Cir-seq) method.

Grants and funding

This work was supported by the German Research Council (SFB 1021 TP A02 to N.B. and S.B. and TP Z02 to J.P.M. and T.H.).